β-carotene biosynthesis in batch, fed batch and continuous submerged culture of yeasts Rhodotorula

Ludmiła Bogacz-Radomska

Abstract

In this PhD thesis research, that concerns the β-carotene biosynthesis in batch, fed batch and continuous submerged culture of yeasts Rhodotorula, has been studied the influence of media composition and chosen physicochemical factors on production rate and productivity of β-carotene biosynthesis. In presented PhD thesis strain Rhodotorula rubra 350 has been reported for β-carotene biosynthesis because of its high production rate and productivity of β-carotene biosynthesis. Growth of β-carotene content is not proportionate to biomass concentration. Media composition and pH of the environment were assessed in submerged batch culture on rotary shaker. Media composition was established based on Box-Behnken design, experimental designs for response surface methodology and with Statistica 10, StatSoft. Based on analyses optimal media composition were obtained (g dm-3): glucose – 60,0; glycerol – 31,1; KH2PO4 – 4,9; ZnCl2 – 0,4; yeast extract – 2,2; (NH4)2SO4 –17,1. Automatically pH regulation on level 5,0 in submerged batch cultures carried in bioreactor has increased production rate of β-carotene biosynthesis. Among applied cultivation methods the highest biosynthesis rate of β-carotene has been achieved in feed batch culture, feeding with full medium containing sources of carbon a micro- and macroelements. Continuous culture based on chemostat model has been studied, the optimal dilution rate equal to 0,0250 h-1. In these cultivations the highest production rate of β-carotene biosynthesis has been achieved. Using the light in the UV range with emission maximum by wavelength equal to 370 nm has increased production rate of β-carotene biosynthesis in submerged batch culture of yeasts Rhodotorula rubra 350 carried in bioreactor
Diploma typeDoctor of Philosophy
Author Ludmiła Bogacz-Radomska (EaE / IChaFT / DBaFA)
Ludmiła Bogacz-Radomska,,
- Department of Biotechnology and Food Analysis
Title in Englishβ-carotene biosynthesis in batch, fed batch and continuous submerged culture of yeasts Rhodotorula
Languagepl polski
Certifying UnitFaculty of Engineering and Economics (EaE)
Disciplinetechnologia żywności i żywienia / nauki rolnicze() / nauki rolnicze, leśne i weterynaryjne()
Defense Date03-07-2015
End date08-07-2015
Supervisor Jerzy Pietkiewicz (IChaFT / KBŻ / ZMB)
Jerzy Pietkiewicz,,
- Zakład Mikrobiologii i Biosyntezy

External reviewers Włodzimierz Bednarski
Włodzimierz Bednarski,,
-

Zdzisław Targoński
Zdzisław Targoński,,
-
Pages152
Keywords in Englishβ-carotene, carotenoids, Rhodotorula, culture methods
Abstract in EnglishIn this PhD thesis research, that concerns the β-carotene biosynthesis in batch, fed batch and continuous submerged culture of yeasts Rhodotorula, has been studied the influence of media composition and chosen physicochemical factors on production rate and productivity of β-carotene biosynthesis. In presented PhD thesis strain Rhodotorula rubra 350 has been reported for β-carotene biosynthesis because of its high production rate and productivity of β-carotene biosynthesis. Growth of β-carotene content is not proportionate to biomass concentration. Media composition and pH of the environment were assessed in submerged batch culture on rotary shaker. Media composition was established based on Box-Behnken design, experimental designs for response surface methodology and with Statistica 10, StatSoft. Based on analyses optimal media composition were obtained (g dm-3): glucose – 60,0; glycerol – 31,1; KH2PO4 – 4,9; ZnCl2 – 0,4; yeast extract – 2,2; (NH4)2SO4 –17,1. Automatically pH regulation on level 5,0 in submerged batch cultures carried in bioreactor has increased production rate of β-carotene biosynthesis. Among applied cultivation methods the highest biosynthesis rate of β-carotene has been achieved in feed batch culture, feeding with full medium containing sources of carbon a micro- and macroelements. Continuous culture based on chemostat model has been studied, the optimal dilution rate equal to 0,0250 h-1. In these cultivations the highest production rate of β-carotene biosynthesis has been achieved. Using the light in the UV range with emission maximum by wavelength equal to 370 nm has increased production rate of β-carotene biosynthesis in submerged batch culture of yeasts Rhodotorula rubra 350 carried in bioreactor
Thesis file
Bogacz_Radomska_L_Biosynteza_β_Karotenu_W_Okresowych.pdf 2,24 MB

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