Oral Administration of Oat Beta-Glucan Preparations of Different Molecular Weight Results in Regulation of Genes Connected with Immune Response in Peripheral Blood of Rats with LPS-Induced Enteritis

Katarzyna Błaszczyk , Małgorzata Gajewska , Jacek Wilczak , Dariusz Kamola , Alicja Majewska , Joanna Harasym , Joanna Gromadzka-Ostrowska

Abstract

Purpose Beta-glucans are biologically active polysaccharides having antioxidant, immunomodulatory, and antiinflammatory properties. This study investigated the transcriptomic profile in peripheral blood of rats with LPS-induced enteritis, which were fed a diet supplemented with high- (G1) and low- (G2) molecular-weight oat beta-glucans. Methods Two-color rat gene expression microarrays were applied and the analysis was performed using a common reference design to provide easy means of comparing samples from various experimental conditions against one another. Common reference sample was labeled with cyanine 3 (Cy3) and investigated samples from each experimental group: C-G0 (control group fed semi-synthetic diet), LPS-G0 (LPS-challenged group fed semi-synthetic diet), LPS-G1 (LPS-challenged group fed G1 beta-glucan enriched diet), and LPS-G2 (LPS-challenged group fed G2 beta-glucan enriched diet) were labeled with cyanine 5 (Cy5). Each microarray was performed in quadruplicate. Statistical analysis was performed using one-way ANOVA and Tukey’s HSD post-hoc test (p<0.05). A multiple testing correction was performed using Benjamini and Hochberg False Discovery Rate<5%. A quantitative real-time RT-PCR was performed to verify the expression of chosen transcripts. Results The microarray analyses revealed differentially expressed transcripts between: the LPS-G0 and the control groups: C-G0 (138 genes), the LPS-G1 and LPS-G0 groups (533 genes), and the LPS-G2 and LPS-G0 groups (97 genes). Several differentially expressed genes in the beta-glucan-supplemented groups encoded proteins belonging to TLR and NLR signaling pathways, as well as prostaglandin synthesis and regulation pathways. Both beta-glucans up-regulated the expression of Atg10, which belongs to the family of autophagy-related genes, suggesting a possible link between autophagy induction and beta-glucan supplementation. Conclusion The changes in gene expression observed in the peripheral blood indicate that oat beta-glucans exerted a protective effect in rats with an induced inflammatory state caused by LPS challenge. The greater number of differentially expressed genes was observed in group supplemented with G1 beta-glucan, pointing at the differences in the mode of action of highand low-molecular-weight beta-glucans in the organism.
Author Katarzyna Błaszczyk - Warsaw University of Life Sciences (SGGW) [Szkoła Główna Gospodarstwa Wiejskiego]
Katarzyna Błaszczyk,,
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, Małgorzata Gajewska - Warsaw University of Life Sciences (SGGW) [Szkoła Główna Gospodarstwa Wiejskiego]
Małgorzata Gajewska,,
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, Jacek Wilczak - Warsaw University of Life Sciences (SGGW) [Szkoła Główna Gospodarstwa Wiejskiego]
Jacek Wilczak,,
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, Dariusz Kamola - Warsaw University of Life Sciences (SGGW) [Szkoła Główna Gospodarstwa Wiejskiego]
Dariusz Kamola,,
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, Alicja Majewska - Warsaw University of Life Sciences (SGGW) [Szkoła Główna Gospodarstwa Wiejskiego]
Alicja Majewska,,
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, Joanna Harasym (EaE / IChaFT / DBaFA)
Joanna Harasym,,
- Department of Biotechnology and Food Analysis
, Joanna Gromadzka-Ostrowska - Warsaw University of Life Sciences (SGGW) [Szkoła Główna Gospodarstwa Wiejskiego]
Joanna Gromadzka-Ostrowska,,
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Journal seriesEuropean Journal of Nutrition, ISSN 1436-6207, e-ISSN 1436-6215, (N/A 100 pkt)
Issue year2019
Vol58
No7
Pages2859-2873
Publication size in sheets0.7
Keywords in EnglishAutophagy, Beta-glucans, Enteritis, Immunomodulation, Transcriptomic Profile
ASJC Classification2701 Medicine (miscellaneous); 2916 Nutrition and Dietetics
DOIDOI:10.1007/s00394-018-1838-3
URL https://link.springer.com/article/10.1007%2Fs00394-018-1838-3
Languageen angielski
File
Blaszczyk_Gajewska_Wilczak_Kamola_Majweska_Harasym_Gromadzka_Ostrowska_Oral_Adminsitration_Of_Oat.pdf 1,2 MB
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.195; WoS Impact Factor: 2018 = 4.449 (2) - 2018=4.076 (5)
Citation count*3 (2019-12-05)
Additional fields
Uwagi:This project was funded by a grant no.: N N312 427440 from the National Science Centre, Poland. We would like to thank Futurum Ltd., Częstochowa, Poland, for support in beta-glucan manufacturing. We also thank KONTEKST Translations company for their language editing service.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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