Anticancer Activity of Oat β-Glucan in Combination with Electroporation on Human Cancer Cells

Anna Choromańska , Julita Kulbacka , Joanna Harasym , Magda Dubińska-Magiera , Jolanta Saczko

Abstract

The currently available data suggest that natural products may exert significant cytotoxic and immunomodulatory effects. Plant-derived chemotherapeutic agents such as taxol, etoposide or vincristine, currently used in cancer therapy, are prominent examples in this regard. However, there is a need for new and natural anticancer compounds with low or without toxicity to normal cells. One of the active compounds responsible for the immune effects is β-glucan derived from cereals, fungi, seaweeds, yeasts and bacteria. The recent data suggest that β-glucans are potent immunomodulators with anticancer properties. Antitumor properties of fungi and yeast derived β-glucans have been widely recognized, but those polysaccharides are mostly insoluble, creating several problems especially in topical formulation. To overcome the issue of low water solubility, in the current study a more soluble β-glucan type from oats was chosen for the investigation of its antitumor activities. Cytotoxic effects were studied using a human melanoma cell line (Me45). The effect of electroporation on the antitumor activity of oat β-glucan was investigated as well. Cellular viability assessment, immunocytochemistry and immunofluochemistry were employed to evaluate biologic effects. Our results indicate strong anticancer properties of oat β-glucan, enhanced by electroporation
Author Anna Choromańska
Anna Choromańska,,
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, Julita Kulbacka
Julita Kulbacka,,
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, Joanna Harasym (EaE / IChaFT / DBaFA)
Joanna Harasym,,
- Department of Biotechnology and Food Analysis
, Magda Dubińska-Magiera
Magda Dubińska-Magiera,,
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, Jolanta Saczko
Jolanta Saczko,,
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Journal seriesActa Poloniae Pharmaceutica -Drug Research, ISSN 0001-6837, e-ISSN 2353-5288, (A 15 pkt)
Issue year2017
Vol74
No2
Pages616-623
Publication size in sheets0.3
Keywords in EnglishOat β-glucan, electroporation, melanoma, apoptosis
ASJC Classification3003 Pharmaceutical Science; 3004 Pharmacology
Languageen angielski
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Choromanska_Kulbacka_Harasym_Dubinska_Saczko_Anticancer_Activity_Of_Oat.pdf 890,79 KB
Score (nominal)15
ScoreMinisterial score = 15.0, 02-10-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.452; WoS Impact Factor: 2017 = 0.531 (2) - 2017=0.884 (5)
Citation count*6 (2019-12-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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