Chemical profiling and cytotoxic activity of 150-year old original sample of Jerusalem Balsam

Jacek Łyczko , Aleksandra Pawlak , Iwo Augustyński , Piotr Okińczyc , Jakub Szperlik , Anna Kulma , Henryk Różański , Bożena Obmińska-Mrukowicz , Antoni Szumny


Herbal formulations have been used in ethnomedicine and pharmacy around the world for thousands of years. One of them is Jerusalem Balsam (JB), which came into use in the seventeenth century. Today, people still produce and use it regularly as prophylactic supplement. JB has been widely used in Europe since the nineteenth century, as a remedy possessing antibacterial, antifungal and anti-inflammatory activities. The composition of the product was not known, although possible formulations were reported. In this study the original sample, which dated back to 1870, was investigated for chemical composition and cytotoxic activity. The obtained results were compared with results from more recently produced samples. Several tests were carried out, namely GC-MS, UPLC-PDA-Q-TOF-MS and MTT. Only the 150-year old sample showed a significant cytotoxic activity on cancer cell lines. At a concentration of 125 μg/mL after 72 h of incubation, the original sample inhibited almost 90% of cell metabolic activity, while contemporary samples showed none or little activity. None of the tested samples showed a significant impact on normal cells. These results may be attributed to the activities of benzoic acid and its derivatives, cinnamic acid derivatives, vanillin, group of sesquiterpenes and cembrene.
Author Jacek Łyczko - Wrocław University of Environmental and Life Sciences (UPWr)
Jacek Łyczko,,
, Aleksandra Pawlak - Wrocław University of Environmental and Life Sciences (UPWr)
Aleksandra Pawlak,,
, Iwo Augustyński (E&F / DEPaERS)
Iwo Augustyński,,
- Department of Economic Policy and European Regional Studies
, Piotr Okińczyc - Wrocław Medical University (UMW)
Piotr Okińczyc,,
, Jakub Szperlik - University of Wrocław (UWr), Wrocław University of Environmental and Life Sciences (UPWr)
Jakub Szperlik,,
, Anna Kulma - University of Wrocław (UWr)
Anna Kulma,,
, Henryk Różański - Państwowa Wyższa Szkoła Zawodowa w Krośnie
Henryk Różański,,
, Bożena Obmińska-Mrukowicz - Wrocław University of Environmental and Life Sciences (UPWr)
Bożena Obmińska-Mrukowicz,,
, Antoni Szumny - Wrocław University of Environmental and Life Sciences (UPWr)
Antoni Szumny,,
Total number of authors10
Journal seriesFood and Chemical Toxicology, ISSN 0278-6915, e-ISSN 1873-6351, (N/A 100 pkt)
Issue year2020
Publication size in sheets0.3
Article number111183
Keywords in EnglishEssential oils, Cytotoxicity, Herbal formulation, Jerusalem Balsam
ASJC Classification2700 General Medicine; 1106 Food Science; 3005 Toxicology
Languageen angielski
Lyczko_Pawlak_Augustynski_Okinczyk_Szperlik_Kulma_Rozanski_Obminska-Mrukowicz_Szumny_Chemical_profiling_and_cytotoxic_activity.pdf 1,19 MB
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.277; WoS Impact Factor: 2018 = 3.775 (2) - 2018=4.248 (5)
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