Pectin from transgenic flax shives regulates extracellular matrix remodelling in human skin fibroblasts

Wioletta Wojtasik , Magdalena Czemplik , Marta Preisner , Lucyna Dymińska , Guo Yuan , Jan Szopa , Anna Kulma

Abstract

Pectin, a polysaccharide polymer from the plant cell wall, is an underestimated natural resource with many potential applications in the food and medical industries. Here we present, for the first time, the chemical composition of pectin obtained from flax shives, a by-product of flax fibre processing. The shives from transgenic flax overexpressing -glucanase were analysed, revealing that genetic modifi- cation caused an increase in content of lignin, hemicellulose and pectin, without changes to cellulose, rearrangement of the structure of pectin and cellulose, a decrease in the content of phenolic compounds associated with the cell wall, and an increase in antioxidant capacity of the pectin CDTA fraction. The influence of pectin extract on the extracellular matrix remodelling process was verified. In fibroblast skin cells with induced oxidative stress, addition of pectin caused a reversal of the decrease of mRNA collagen genes, an increase of matrix metalloproteinase, interleukin 6 and MCP-1 gene expression, and a reduction in levels of TIMP-1 and SOCS-1 mRNA. The obtained results, in particular strong antioxidant properties of flax shives pectin from the CDTA-soluble fraction and its significant influence on genes participating in extracellular matrix remodelling, suggest the possible application of flax shives pectin in the wound healing process.
Author Wioletta Wojtasik - University of Wrocław (UWr)
Wioletta Wojtasik,,
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, Magdalena Czemplik - University of Wrocław (UWr)
Magdalena Czemplik,,
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, Marta Preisner - University of Wrocław (UWr)
Marta Preisner,,
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, Lucyna Dymińska (EaE / IChaFT / DBCh)
Lucyna Dymińska,,
- Department of Bioorganic Chemistry
, Guo Yuan - Chinese Academy of Agricultural Sciences (CAAS)
Guo Yuan,,
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, Jan Szopa - University of Wrocław (UWr)
Jan Szopa,,
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, Anna Kulma - University of Wrocław (UWr)
Anna Kulma,,
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Journal seriesProcess Biochemistry, ISSN 1359-5113, (A 30 pkt)
Issue year2017
No55
Pages187-198
Publication size in sheets0.55
Keywords in EnglishPectin, Flax, Shives, Phenolic compounds, Extracellular matrix remodelling, Fibroblast
ASJC Classification2402 Applied Microbiology and Biotechnology; 1303 Biochemistry; 1502 Bioengineering
DOIDOI:10.1016/j.procbio.2017.02.001
Languageen angielski
Score (nominal)30
Score sourcejournalList
Publication indicators Scopus Citations = 1; WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.075; WoS Impact Factor: 2017 = 2.616 (2) - 2017=2.987 (5)
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