Pro-apoptotic properties of (1,3)(1,4)-β-d-glucan from Avena sativa on human melanoma HTB-140 cells in vitro
Andrzej Parzonko , Magdalena Makarewicz-Wujec , Edyta Jaszewska , Joanna Harasym , Małgorzata Kozłowska-Wojciechowska
AbstractIn this study, the growth-inhibitory effect of polysaccharide (1,3)(1,4)-β-d-glucan from oat, Avena sativa L. grains was explored on the human skin melanoma HTB-140 cells in vitro. The oat β-d-glucan (OBG) exerted cytotoxic action on HTB-140 cells. After 24 h of incubation, LD50 (concentration at which 50% of the cells were found dead) was obtained of 194.6 ± 9.8 μg/mL. The oat β-d-glucan caused a concentration-dependent increase of caspase-3/-7 activation and appearance of phosphatidylserine on the external surface of cellular membranes where it was bound to annexin V-FITC, demonstrating the induction of apoptosis. Intracellular ATP level decreased along with the mitochondrial potential, which suggested a mitochondrial pathway of apoptosis. A cell cycle analysis showed increase in the number of apoptotic cells, increase in the number of cells in G1 phase and decrease in the number of cells in G2/M. Although the detailed mechanism for the anti-tumor activity of the oat β-d-glucan still needs further investigation, this study provides preliminary insights into this direction along with perspectives of developing it as an anti-tumor agent.
|Journal series||International Journal of Biological Macromolecules, ISSN 0141-8130, (A 35 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||(1,3)(1,4)-β-d-Glucan, Avena sativa, Apoptosis|
|ASJC Classification||; ; ;|
|Publication indicators||= 19; : 2015 = 1.303; : 2015 = 3.138 (2) - 2015=3.22 (5)|
|Citation count*||24 (2019-12-14)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.