Pro-apoptotic properties of (1,3)(1,4)-β-d-glucan from Avena sativa on human melanoma HTB-140 cells in vitro

Andrzej Parzonko , Magdalena Makarewicz-Wujec , Edyta Jaszewska , Joanna Harasym , Małgorzata Kozłowska-Wojciechowska

Abstract

In this study, the growth-inhibitory effect of polysaccharide (1,3)(1,4)-β-d-glucan from oat, Avena sativa L. grains was explored on the human skin melanoma HTB-140 cells in vitro. The oat β-d-glucan (OBG) exerted cytotoxic action on HTB-140 cells. After 24 h of incubation, LD50 (concentration at which 50% of the cells were found dead) was obtained of 194.6 ± 9.8 μg/mL. The oat β-d-glucan caused a concentration-dependent increase of caspase-3/-7 activation and appearance of phosphatidylserine on the external surface of cellular membranes where it was bound to annexin V-FITC, demonstrating the induction of apoptosis. Intracellular ATP level decreased along with the mitochondrial potential, which suggested a mitochondrial pathway of apoptosis. A cell cycle analysis showed increase in the number of apoptotic cells, increase in the number of cells in G1 phase and decrease in the number of cells in G2/M. Although the detailed mechanism for the anti-tumor activity of the oat β-d-glucan still needs further investigation, this study provides preliminary insights into this direction along with perspectives of developing it as an anti-tumor agent.
Author Andrzej Parzonko - [Medical University of Warsaw]
Andrzej Parzonko,,
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, Magdalena Makarewicz-Wujec - [Medical University of Warsaw]
Magdalena Makarewicz-Wujec,,
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, Edyta Jaszewska - [Medical University of Warsaw]
Edyta Jaszewska,,
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, Joanna Harasym (EaE / IChaFT / KBŻ)
Joanna Harasym,,
- Katedra Biotechnologii Żywności
, Małgorzata Kozłowska-Wojciechowska - [Medical University of Warsaw]
Małgorzata Kozłowska-Wojciechowska,,
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Journal seriesInternational Journal of Biological Macromolecules, ISSN 0141-8130, (A 35 pkt)
Issue year2015
No72
Pages757-763
Publication size in sheets0.5
Keywords in English(1,3)(1,4)-β-d-Glucan, Avena sativa, Apoptosis
ASJC Classification1312 Molecular Biology; 2700 General Medicine; 1303 Biochemistry; 1315 Structural Biology
DOIDOI:10.1016/j.ijbiomac.2014.09.033
URL http://www.sciencedirect.com/science/article/pii/S0141813014006357
Languageen angielski
File
Pro_apoptotic_properties_of.pdf 1,78 MB
Score (nominal)35
Score sourcejournalList
Publication indicators Scopus Citations = 19; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 1.303; WoS Impact Factor: 2015 = 3.138 (2) - 2015=3.22 (5)
Citation count*24 (2019-12-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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