Anticancer properties of low molecular weight oat beta-glucan – An in vitro study
Anna Choromańska , Julita Kulbacka , Nina Rembiałkowska , Justyna Piłat , Remigiusz Olędzki , Joanna Harasym , Jolanta Saczko
AbstractAnticancer properties of 1–3, 1–4 oat beta glucan are under intensive investigation now. Antitumor characteristic of fungi and yeast beta-glucans have been widely recognized, but those polysaccharides are mostly insoluble which creates several problems especially in topical formulation. Also high molecular weight oat beta-glucans reveal high viscosity which restricts its application. According to those problems in the current study the antitumor activities of low molecular weight beta-glucan derived from oats were investigated in cancer cells: Me45, A431 and normal HaCaT and murine macrophages P388/D1. The low molecular weight beta-glucan from oat significantly deceased cancer cells viability, while for the normal cells it was non-toxic. It was observed that with the increasing incubation time and the beta-glucan concentration the cancer cells viability significantly deceased. Furthermore for the normal cells the low molecular weight beta-glucan from oat was non-toxic. Immunocytochemical ABC analysis showed that beta-glucan induced strong expression of caspase-12 in both cancer cell lines, while in HaCaT cells ABC reaction was significantly lower and in P388/D1 cell line ABC reaction was negative. Our preliminary studies show strong anti-tumor properties of new low molecular weight beta-glucan from oat and at the same time no toxicity for normal cells.
|Journal series||International Journal of Biological Macromolecules, ISSN 0141-8130, (A 35 pkt)|
|Publication size in sheets||0.4|
|Keywords in English||Oat beta-glucan, Melanoma, Skin cancer|
|ASJC Classification||; ; ;|
|Publication indicators||= 37; : 2015 = 1.303; : 2015 = 3.138 (2) - 2015=3.22 (5)|
|Citation count*||51 (2019-12-05)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.