Anticancer properties of low molecular weight oat beta-glucan – An in vitro study

Anna Choromańska , Julita Kulbacka , Nina Rembiałkowska , Justyna Piłat , Remigiusz Olędzki , Joanna Harasym , Jolanta Saczko


Anticancer properties of 1–3, 1–4 oat beta glucan are under intensive investigation now. Antitumor characteristic of fungi and yeast beta-glucans have been widely recognized, but those polysaccharides are mostly insoluble which creates several problems especially in topical formulation. Also high molecular weight oat beta-glucans reveal high viscosity which restricts its application. According to those problems in the current study the antitumor activities of low molecular weight beta-glucan derived from oats were investigated in cancer cells: Me45, A431 and normal HaCaT and murine macrophages P388/D1. The low molecular weight beta-glucan from oat significantly deceased cancer cells viability, while for the normal cells it was non-toxic. It was observed that with the increasing incubation time and the beta-glucan concentration the cancer cells viability significantly deceased. Furthermore for the normal cells the low molecular weight beta-glucan from oat was non-toxic. Immunocytochemical ABC analysis showed that beta-glucan induced strong expression of caspase-12 in both cancer cell lines, while in HaCaT cells ABC reaction was significantly lower and in P388/D1 cell line ABC reaction was negative. Our preliminary studies show strong anti-tumor properties of new low molecular weight beta-glucan from oat and at the same time no toxicity for normal cells.
Author Anna Choromańska - Wrocław Medical University (UMW)
Anna Choromańska,,
, Julita Kulbacka - Wrocław Medical University (UMW)
Julita Kulbacka,,
, Nina Rembiałkowska - [Wrocław Medical University (UMW)]
Nina Rembiałkowska,,
- Uniwersytet Medyczny im. Piastów Śląskich we Wrocławiu
, Justyna Piłat - [Wrocław Medical University (UMW)]
Justyna Piłat,,
- Uniwersytet Medyczny im. Piastów Śląskich we Wrocławiu
, Remigiusz Olędzki (EaE / IChaFT / KBŻ)
Remigiusz Olędzki,,
- Katedra Biotechnologii Żywności
, Joanna Harasym (EaE / IChaFT / KBŻ)
Joanna Harasym,,
- Katedra Biotechnologii Żywności
, Jolanta Saczko - Wrocław Medical University (UMW)
Jolanta Saczko,,
Journal seriesInternational Journal of Biological Macromolecules, ISSN 0141-8130, (A 35 pkt)
Issue year2015
Publication size in sheets0.4
Keywords in EnglishOat beta-glucan, Melanoma, Skin cancer
ASJC Classification1312 Molecular Biology; 2700 General Medicine; 1303 Biochemistry; 1315 Structural Biology
Languageen angielski
Anticancer_properties_of_low_molecular_weight_oat_beta_glucan_2015.pdf 1,34 MB
Score (nominal)35
Score sourcejournalList
Publication indicators Scopus Citations = 37; WoS Citations = 37; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 1.303; WoS Impact Factor: 2015 = 3.138 (2) - 2015=3.22 (5)
Citation count*60 (2020-07-10)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.