Anticancer properties of low molecular weight oat beta-glucan – An in vitro study

Anna Choromańska , Julita Kulbacka , Nina Rembiałkowska , Justyna Piłat , Remigiusz Olędzki , Joanna Harasym , Jolanta Saczko

Abstract

Anticancer properties of 1–3, 1–4 oat beta glucan are under intensive investigation now. Antitumor characteristic of fungi and yeast beta-glucans have been widely recognized, but those polysaccharides are mostly insoluble which creates several problems especially in topical formulation. Also high molecular weight oat beta-glucans reveal high viscosity which restricts its application. According to those problems in the current study the antitumor activities of low molecular weight beta-glucan derived from oats were investigated in cancer cells: Me45, A431 and normal HaCaT and murine macrophages P388/D1. The low molecular weight beta-glucan from oat significantly deceased cancer cells viability, while for the normal cells it was non-toxic. It was observed that with the increasing incubation time and the beta-glucan concentration the cancer cells viability significantly deceased. Furthermore for the normal cells the low molecular weight beta-glucan from oat was non-toxic. Immunocytochemical ABC analysis showed that beta-glucan induced strong expression of caspase-12 in both cancer cell lines, while in HaCaT cells ABC reaction was significantly lower and in P388/D1 cell line ABC reaction was negative. Our preliminary studies show strong anti-tumor properties of new low molecular weight beta-glucan from oat and at the same time no toxicity for normal cells.
Author Anna Choromańska - [Wroclaw Medical University]
Anna Choromańska,,
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, Julita Kulbacka - [Wroclaw Medical University]
Julita Kulbacka,,
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, Nina Rembiałkowska - [Wroclaw Medical University]
Nina Rembiałkowska,,
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, Justyna Piłat - [Wroclaw Medical University]
Justyna Piłat,,
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, Remigiusz Olędzki (EaE / IChaFT / KBŻ)
Remigiusz Olędzki,,
- Katedra Biotechnologii Żywności
, Joanna Harasym (EaE / IChaFT / KBŻ)
Joanna Harasym,,
- Katedra Biotechnologii Żywności
, Jolanta Saczko - [Wroclaw Medical University]
Jolanta Saczko,,
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Journal seriesInternational Journal of Biological Macromolecules, ISSN 0141-8130, (A 35 pkt)
Issue year2015
Vol80
Pages23-28
Publication size in sheets0.4
Keywords in EnglishOat beta-glucan, Melanoma, Skin cancer
ASJC Classification1312 Molecular Biology; 2700 General Medicine; 1303 Biochemistry; 1315 Structural Biology
DOIDOI:10.1016/j.ijbiomac.2015.05.035
URL http://www.sciencedirect.com/science/article/pii/S0141813015003761
Languageen angielski
File
Anticancer_properties_of_low_molecular_weight_oat_beta_glucan_2015.pdf 1,34 MB
Score (nominal)35
Score sourcejournalList
Publication indicators Scopus Citations = 37; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 1.303; WoS Impact Factor: 2015 = 3.138 (2) - 2015=3.22 (5)
Citation count*51 (2019-12-05)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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