The effect of low or high molecular weight oat beta-glucans on the inflammatory and oxidative stress status in the colon of rats with LPS-induced enteritis

Jacek Wilczak , Katarzyna Błaszczyk , Dariusz Kamola , M. Gajewska , Joanna Harasym , M. Jałosińska , Sylwia Gudej , Dominika Suchecka , Michał Oczkowski , Joanna Gromadzka-Ostrowska

Abstract

The aim of the study was to investigate the protective effect of low and high molecular weight beta-glucans on the chosen immunological parameters, markers of antioxidative potential in rats’ colon tissue, the number of lactic acid bacteria (LAB) and the concentration of short-chain fatty acids (SCFA) in rats’ faeces. Methods: The experiment was carried out on 72 8-week old male Sprague–Dawley rats: control (n = 36) and experimental (n = 36). In half of the animals from each group enteritis was induced by LPS (10 mg kg−1 ). Rats from the experimental group were divided into two groups receiving high (GI) or low (GII) molecular weight beta-glucans for 6 consecutive weeks. Results: LPS evoked enteritis in all the treated animals, manifested by changes in the levels of IL-10, IL-12 and TNF-alpha, as well as in the number of intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) in the colon tissue. Dietary supplementation with beta-glucans following LPS treatment partially reversed this effect. The changes in SCFA concentration were noted, indicating an improvement of the fermentation process in the colon. This effect coincided with an increased number of LAB, pointing at the prebiotic properties of beta-glucans. The positive influence of beta-glucans was also manifested by the improved values of antioxidative potential markers (TAS, SOD, GR and GPx activity, TBARS concentration), noted especially in rats with LPS-induced enteritis. This influence was more pronounced in the case of low molecular weight oat beta-glucan (GII). Conclusions: The present study showed a positive effect of beta-glucans, especially the low molecular weight form, on the colon tissue of healthy rats, as well as animals with LPS-induced enteritis
Author Jacek Wilczak - Warsaw University of Life Sciences (SGGW)
Jacek Wilczak,,
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, Katarzyna Błaszczyk - Warsaw University of Life Sciences (SGGW)
Katarzyna Błaszczyk,,
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, Dariusz Kamola - Warsaw University of Life Sciences (SGGW)
Dariusz Kamola,,
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, M. Gajewska - [Warsaw University of Life Sciences (SGGW)]
M. Gajewska,,
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- Szkoła Główna Gospodarstwa Wiejskiego
, Joanna Harasym (EaE / IChaFT / KBŻ)
Joanna Harasym,,
- Katedra Biotechnologii Żywności
, M. Jałosińska - [Warsaw University of Life Sciences (SGGW)]
M. Jałosińska,,
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- Szkoła Główna Gospodarstwa Wiejskiego
, Sylwia Gudej - Warsaw University of Life Sciences (SGGW)
Sylwia Gudej,,
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, Dominika Suchecka - Warsaw University of Life Sciences (SGGW)
Dominika Suchecka,,
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, Michał Oczkowski - Warsaw University of Life Sciences (SGGW)
Michał Oczkowski,,
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, Joanna Gromadzka-Ostrowska - Warsaw University of Life Sciences (SGGW)
Joanna Gromadzka-Ostrowska,,
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Journal seriesFood and Function, ISSN 2042-6496, e-ISSN 2042-650X, (A 30 pkt)
Issue year2015
No6 (2)
Pages590-603
Publication size in sheets0.65
ASJC Classification2700 General Medicine; 1106 Food Science
DOIDOI:10.1039/C4FO00638K
URL http://pubs.rsc.org/en/content/articlelanding/2015/fo/c4fo00638k#!divAbstract
Languageen angielski
Score (nominal)30
Score sourcejournalList
Publication indicators Scopus Citations = 22; WoS Citations = 23; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 1.02; WoS Impact Factor: 2015 = 2.686 (2) - 2015=3.178 (5)
Citation count*35 (2020-07-10)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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