Immobilization of Naringinase from Aspergillus Niger on a Magnetic Polysaccharide Carrier

Joanna Bodakowska-Boczniewicz , Zbigniew Garncarek

Abstract

Naringinase is an enzymatic complex used in the deglycosylation of compounds with a high application potential in the food and pharmaceutical industries. The aim of the study was to immobilize naringinase from Aspergillus niger KMS on a magnetic carrier obtained on the basis of carob gum activated by polyethyleneimine. Response surface methodology was used to optimize naringinase immobilization taking into account the following factors: pH, immobilization time, initial concentration of naringinase and immobilization temperature. The adsorption of the enzyme on a magnetic carrier was a reversible process. The binding force of naringinase was increased by crosslinking the enzyme with the carrier using dextran aldehyde. The crosslinked enzyme had better stability in an acidic environment and at a higher temperature compared to the free form. The immobilization and stabilization of naringinase by dextran aldehyde on the magnetic polysaccharide carrier lowered the activation energy, thus increasing the catalytic capacity of the investigated enzyme and increasing the activation energy of the thermal deactivation process, which confirms higher stability of the immobilized enzyme in comparison with free naringinase. The preparation of crosslinked naringinase retained over 80% of its initial activity after 10 runs of naringin hydrolysis from fresh and model grapefruit juice.
Author Joanna Bodakowska-Boczniewicz (WUE)
Joanna Bodakowska-Boczniewicz,,
- Wrocław University of Economics
, Zbigniew Garncarek (PE / DBFA)
Zbigniew Garncarek,,
- Department of Biotechnology and Food Analysis
Journal seriesMolecules, ISSN 1420-3049, (N/A 100 pkt)
Issue year2020
Vol25
No12
Pages1-25
Publication size in sheets1.2
Article number2731
Keywords in Englishnaringinase; immobilization; magnetic carrier; dextran aldehyde
ASJC Classification1605 Organic Chemistry
DOIDOI:10.3390/molecules25122731
Languageen angielski
File
Bodakowska-Boczniewicz_J_Garncarek_Z_Immobilization_of_Naringinase_from_Aspergillus.pdf 3,61 MB
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.146; WoS Impact Factor: 2018 = 3.06 (2) - 2018=3.38 (5)
Citation count*
Additional fields
UwagaThis research was funded by the Wroclaw University of Economics and Business
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back