Time-dependent indirect antioxidative effects of oat beta-glucans on peripheral blood parameters in the animal model of colon inflammation

Łukasz Kopiasz , Katarzyna Dziendzikowska , Małgorzata Gajewska , Jacek Wilczak , Joanna Harasym , Ewa Żyła , Dariusz Kamola , Michał Oczkowski , Tomasz Królikowski , Joanna Gromadzka-Ostrowska

Abstract

Oat beta-glucans are polysaccharides, belonging to soluble fiber fraction, that show a wide spectrum of biological activity. The aim of this study was to evaluate the time-dependent antioxidative effect of chemically pure oat beta-glucan fractions, characterized by different molar mass, which were fed to animals with early stage of 2,4,6-trinitrobenzene sulfonic acid (TNBS) - induced colitis. Methods: The study was conducted on 150 adult male Sprague Dawley rats assigned to two groups—healthy control (H) and colitis (C) with colon inflammation induced by per rectum administration of TNBS. The animals from both groups were divided into 3 nutritional subgroups, receiving for 3, 7 or 21 days AIN-93M feed without beta-glucan (βG−) or with 1% (w/w) low molar mass oat beta-glucan (βGl+) or 1% (w/w) high molar mass oat beta-glucan (βGh+). After 3, 7 and 21 days, the animals were euthanized, peripheral blood was collected from the heart for further analysis. Results: The results of analyses performed on blood samples showed small changes in lymphocytes count and red blood cell parameters such as the number of red blood cell, mean corpuscular hemoglobin concentration and mean corpuscular volume (RBC, MCHC, MCV respectively) as well as normalization of antioxidant potential accompanying moderate inflammatory state of colon mucosa and submucosa. Conclusion: Oat beta-glucans exert an indirect antioxidant effect in animals with TNBS-induced colitis, with greater effectiveness in removing systemic effects of colon inflammation found for low molar mass oat beta-glucan.
Author Łukasz Kopiasz - Warsaw University of Life Sciences (SGGW)
Łukasz Kopiasz,,
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, Katarzyna Dziendzikowska - Warsaw University of Life Sciences (SGGW)
Katarzyna Dziendzikowska,,
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, Małgorzata Gajewska - Warsaw University of Life Sciences (SGGW)
Małgorzata Gajewska,,
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, Jacek Wilczak - Warsaw University of Life Sciences (SGGW)
Jacek Wilczak,,
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, Joanna Harasym (PE / DBFA)
Joanna Harasym,,
- Department of Biotechnology and Food Analysis
, Ewa Żyła - Warsaw University of Life Sciences (SGGW)
Ewa Żyła,,
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, Dariusz Kamola - Warsaw University of Life Sciences (SGGW)
Dariusz Kamola,,
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, Michał Oczkowski - Warsaw University of Life Sciences (SGGW)
Michał Oczkowski,,
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, Tomasz Królikowski - Warsaw University of Life Sciences (SGGW)
Tomasz Królikowski,,
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, Joanna Gromadzka-Ostrowska - Warsaw University of Life Sciences (SGGW)
Joanna Gromadzka-Ostrowska,,
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Journal seriesAntioxidants, ISSN 2076-3921, e-ISSN 2076-3921, (N/A 100 pkt)
Issue year2020
Vol9
No5
Pages1-22
Publication size in sheets1.05
Article number375
Keywords in English oat beta-glucan; peripheral blood; colitis; oxidative stress; rats
ASJC Classification1303 Biochemistry; 1307 Cell Biology; 1308 Clinical Biochemistry; 1312 Molecular Biology; 1314 Physiology
DOIDOI:10.3390/antiox9050375
Languageen angielski
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Kopiasz_Dziendzikowska_ Gajewska_Wilczak_Harasym_Zyla_Kamola_Oczkowski_Krolikowski_Gromadzka-Ostrowska_Time-Dependent_Indirect Antioxidative_ Effects2020.pdf 1,55 MB
Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.439
Citation count*
Additional fields
UwagaThe authors acknowledge the financial support of the National Science Centre, Poland through grantnumber2015/17/B/NZ9/01740
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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