Screening of medium components and process parameters for sugar beet molasses vinasse decolorization by Lactobacillus plantarum using Plackett-Burman experimental design

Przemysław Seruga , Małgorzata Krzywonos


Sugar beet molasses vinasse is characterized by a high COD and dark brown color produced by three groups of colorants: melanoidins, caramels, and invert degradation products of alkaline hydrolysis. These environmental pollutants have to be effectively removed via biodegradation processes before discharge into a watercourse. The aim of this study was to assess how the type of the bacterial culture used (static or agitated), and the addition of nutrients (wheat stillage, MgSO4·7H2O, (NH4)2SO4, KH2PO4, yeast extract, glucose, fructose, sucrose, and peptone) contribute to the effectiveness of sugar beet molasses vinasse decolorization with Lactobacillus plantarum. The Plackett-Burman experimental design was used to reduce the number of experiments. Decolorization of nutrient-enriched vinasse (25% v/v) was performed at an initial pH of 6.25 and 37ºC for 7 days. Color was measured spectrophotometrically at 475 nm. Concentrations of sugars, organic acids, and colorants were determined by HPLC. The nutrient was screened when the probability value exceeded 95% of the confidence level. 67% efficiency of decolorization of sugar beet molasses vinasse by L. plantarum was obtained. The type of bacterial culture and the addition of wheat stillage, yeast extract, glucose, peptone, and (NH4)2SO4 were significant factors for the decolorization process.
Author Przemysław Seruga (EaE / IChaFT / DBE)
Przemysław Seruga,,
- Department of Bioprocessing Engineering
, Małgorzata Krzywonos (EaE / IChaFT / DBE)
Małgorzata Krzywonos,,
- Department of Bioprocessing Engineering
Journal seriesPolish Journal of Environmental Studies, ISSN 1230-1485, e-ISSN 2083-5906, (A 15 pkt)
Issue year2015
Publication size in sheets0.5
Keywords in Englishdecolorization, vinasse, melanoidins, Lactobacillus plantarum, Plackett-Burman experimental design
ASJC Classification2300 General Environmental Science; 2304 Environmental Chemistry
Languageen angielski
Seruga_Krzywonos_Screening_of_medium_components_and_process.pdf 70,46 KB
Score (nominal)15
Score sourcejournalList
ScoreMinisterial score = 15.0, 26-11-2019, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 26-11-2019, ArticleFromJournal
Publication indicators Scopus Citations = 6; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 0.689; WoS Impact Factor: 2015 = 0.79 (2) - 2015=0.877 (5)
Citation count*11 (2020-07-11)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.